Normal
Frame	Start codon (ATG)	Stop codon (TGA TAG TAA)	ORF (Start:Stop)
1	376 427 1480	340 343
2	161 434 536 863 968 1163	377 425 428 1286 1328 1454 1589 1655 1769	161:377 434:1286 536:1286 863:1286 968:1286 1163:1286
3	102 246 339 342 345	264 267 474 534 825 861 969 1023 1149 1272	102:264 339:474 342:474 345:474
Complementary
Frame	Start codon (ATG)	Stop codon (TGA TAG TAA)	ORF (Start:Stop)
1	1179 1164 1083 1041 594 435	1110 669 567 339 285 246	1179:1110 1083:669 1041:669 435:339
2	1652 1532 1481	1313 677 641 593	1652:1313 1532:1313 1481:1313
3	544 247 202 103	1600 1534 1528 1492 1462 1408

Numbers denote the 1st-letter position of each codon. Credit for using this program must be given in your publication.

LOCUS       STMKAN       1794 bp    DNA             BCT       17-APR-1992
DEFINITION  S.griseus kan gene encoding aminoglycoside 3-N-acetyltransferase.
ACCESSION   D00681
NID         g217013
KEYWORDS    aminoglycoside 3-N-acetyltransferase; kan gene.
SOURCE      S.griseus (strain SS-1198PR) genomic DNA.
  ORGANISM  Streptomyces griseus
            Eubacteria; Firmicutes; Actinomycetes; Streptomycetes;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ishikawa,J. and Hotta,K.
  TITLE     Nucleotide sequence and transcriptional start point of the kan gene
            encoding an aminoglycoside 3-N-acetyltransferase from Streptomyces
            griseus SS-1198PR
  JOURNAL   Gene 108, 127-132 (1991)
  MEDLINE   92104494
COMMENT     These data kindly submitted in computer readable form by: Jun
            Ishikawa  
            Department of Antibiotics  
            National Institute of Health  
            2-10-35 Kamiosaki  
            Shinagawa-ku  
            Tokyo 141  
            Japan  
            Phone:  03-3444-2181  
            Fax:    03-3446-6286.
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Streptomyces griseus"
     -10_signal      338..343
     mRNA            350..>1288
                     /note="mRNA"
     CDS             434..1288
                     /gene="kan"
                     /codon_start=1
                     /product="aminoglycoside 3-N-acetyltransferase"
                     /db_xref="PID:g217014"
                     /transl_table=11
                     /translation="MDETELLRRSDGPVTRDRIRHDLAALGLVPGDTVMFHTRLSAIG
                     YVSGGPQTVIDALLDVVGPTGTLLVTCGWNDAPPYDFTDWPPAWQEAVRAHHPAFDPR
                     TSEAEHANGRLPEALRRRPGAVRSRHPDVSLAALGASAPALMDAHPWDDPHGPGSPLA
                     RLVALGGRVLLLGAPRDTMTLLHHAEALAQAPGKRFVTYEQPIEVAGERVWRTFRDID
                     SEHGAFDYSSAVPEGQDPFAVIVGSMLAAGIGREGFVGAARSRLFDAAPAVEFGVRWI
                     EEHLNRDR"
BASE COUNT      247 a    683 c    621 g    243 t
ORIGIN      
        1 agatctccac ctcggccttc caggcggcga acgcctcggc cccggtcgag gcgctcgcgt
       61 cgtacgccgc ctggaagtcg aagggctctg cggaccacac catgggggcc gccccgtcct
      121 cgaacacccg ccggaaccag gcgcgttcca cttccgccag atgccgcacc agagccagca
      181 gcgacaaggt ggacggcggc atcgaccgct cgcgcaacgc gtcgtcgtcc agccccgcgc
      241 acttcatggc gagggtctcc cgctgatagt cgagaaaggc cctcaacgtc tcgcgctcgc
      301 cgcccagcag cggaggcgcg atccgttcgt cgtccgttat gatgatggtc cccagtccgt
      361 gttccggccg acagtatgac cggtcgacgt ccgcccggcc cgatccggac gacagctccc
      421 gaggtgatga ggcatggacg agacggaact gctgcgacgc tccgacgggc ccgtgacccg
      481 ggaccggatc cggcacgacc tggccgcgct cggcctcgtc ccgggcgaca ccgtgatgtt
      541 ccatacgcgg ctgtccgcga tcggctacgt ctccggaggc ccccagaccg tcatcgacgc
      601 cctgctggac gtggtgggac cgaccggcac tctgttggtc acctgcggct ggaacgacgc
      661 tccgccctac gacttcaccg actggcctcc cgcctggcag gaggccgtac gcgcccacca
      721 ccccgcgttc gacccgcgga cgagcgaggc cgagcacgcc aacggccgcc ttccggaggc
      781 cctgcgccgc agaccggggg ccgtacgcag tcgccacccc gacgtgagtc tcgcggcgct
      841 cggcgcctcg gcccccgctc tgatggacgc ccacccctgg gacgatccgc acggtcccgg
      901 cagcccgctg gcgcgcctgg tcgccctcgg cggccgggtg ctgctgctcg gcgcgccccg
      961 ggacacgatg acgctgctgc accacgccga ggcgctggcc caggcccccg gcaagcggtt
     1021 cgtgacgtac gagcagccca tcgaggtggc gggcgagcgg gtctggcgca ccttccggga
     1081 catcgactcc gagcacggtg cgttcgacta ctcctcggcc gtgcccgagg ggcaggaccc
     1141 cttcgcggtg atcgtcggtt ccatgctcgc cgcgggcatc ggacgggagg gcttcgtcgg
     1201 ggcggccagg agccggctgt tcgacgccgc cccggccgtc gagttcggcg tccgctggat
     1261 cgaggagcac ctgaaccggg accgctgaac cgggccgccc gacgcccggt tcaggacagc
     1321 cgcaccttga ccgtctgcgt cgccccgcgc gtcccggaga ggtcgccgaa gacgaggcgc
     1381 agcgacgccc ccgtggccgc cgaggtcacg gtcgccggct tcgacacgac cgaggcgacg
     1441 gcccggttcc aggtgagggt caggctggtg cgcgcccgca tggggtcgct cacgcacacc
     1501 gtcgccgtcc cgtcggcgtg ctcggtcacc atcaccgagc acggggcgtc cgccaccagc
     1561 ggcccgacgg tcccgccgaa ccagaagttg accgcggtca ggcgcagcga gggcaccgac
     1621 accccctgct gggcgtccgt gttggcgagc atcctgagcc agccgctgtc cgccgcccgt
     1681 gccgccgtgc gctgtgggga cgcgccgggc aggagttggt acgcgtacgt cgcgtccgtc
     1741 gggtcctggc cgtggtcgaa ccagagggtg aggtacttcc gggagaccgg atcc
//