| Normal | |||
| Frame | Start codon (ATG) | Stop codon (TGA TAG TAA) | ORF (Start:Stop) |
| 1 | 259 496 508 526 532 547 586 727 883 1054 1120 1150 1177 1210 1495 1618 1960 2074 2158 2386 2770 3259 3298 3310 3319 3574 3742 | 250 307 379 394 415 487 1561 1615 1639 1672 1744 1816 1858 1906 1993 2467 2680 2728 2860 3250 | 496:1561 508:1561 526:1561 532:1561 547:1561 586:1561 727:1561 883:1561 1054:1561 1120:1561 1150:1561 1177:1561 1210:1561 1495:1561 2074:2467 2158:2467 2386:2467 2770:2860 |
| 2 | 191 215 320 512 2654 2840 2900 2942 3068 3347 3737 3746 | 260 278 296 482 527 533 626 977 1073 1163 1259 1274 1496 1664 1967 1985 2108 2132 2495 2570 2744 2828 3311 3320 3338 3353 3377 3437 3443 3449 3455 3569 3575 3647 3701 | 191:260 320:482 2654:2744 2840:3311 2900:3311 2942:3311 3068:3311 |
| 3 | 69 339 417 516 1743 1860 1878 1992 2052 2196 2244 2301 2334 2466 2490 2505 2610 2679 3045 | 93 192 444 522 558 735 1182 1584 1593 1626 1680 1701 2640 2886 2913 2937 3069 3117 3156 3240 3396 3489 3504 | 339:444 1743:2640 1860:2640 1878:2640 1992:2640 2052:2640 2196:2640 2244:2640 2301:2640 2334:2640 2466:2640 2490:2640 2505:2640 2679:2886 |
| Complementary | |||
| Frame | Start codon (ATG) | Stop codon (TGA TAG TAA) | ORF (Start:Stop) |
| 1 | 3738 2943 2925 2841 1848 1794 513 321 147 | 3444 3438 3216 3204 2829 2817 2781 2610 2133 1977 1965 1947 1665 903 627 291 | 3738:3444 2943:2829 2925:2829 1848:1665 1794:1665 513:291 |
| 2 | 3098 2387 2159 1388 1211 884 833 740 509 173 29 | 3494 3476 3347 3152 2942 2924 1907 1793 1724 1640 1616 1562 1406 1196 1109 1091 1088 959 911 488 449 350 308 191 167 152 26 | 3098:2942 2387:1907 2159:1907 1388:1196 884:488 833:488 740:488 |
| 3 | 3016 2707 2506 2467 2302 2053 1891 1744 1582 676 | 3742 3697 3676 3574 3532 3505 3397 3256 3097 2992 2674 2641 2512 2188 2179 1681 1669 1627 1585 1495 1489 1390 1021 883 835 820 742 580 493 160 115 94 46 | 2506:2188 2467:2188 2302:2188 2053:1681 1891:1681 1744:1681 1582:1495 676:580 |
LOCUS MSG32KDAP 3762 bp DNA BCT 08-AUG-1996
DEFINITION Mycobacterium bovis genes for 32kDa protein (antigen 85 A) and
MPB51, complete cds.
ACCESSION D26486
NID g804882
KEYWORDS 32kDa protein (antigen 85 A); MPB51; major secreted protein
antigen; fibronectin binding 85 complex-related secreted protein.
SOURCE Mycobacterium bovis (strain:BCG Tokyo) DNA.
ORGANISM Mycobacterium bovis
Eubacteria; Firmicutes; Actinomycetes; Mycobacteria;
Mycobacteriaceae; Mycobacterium.
REFERENCE 1 (bases 1 to 3762)
AUTHORS Ohara,N.
TITLE Direct Submission
JOURNAL Submitted (13-JAN-1994) to the DDBJ/EMBL/GenBank databases. Naoya
Ohara, School of Dentistry, Nagasaki University, Department of Oral
Bacteriology; Sakamoto 1-7-1, Nagasaki, Nagasaki 852, Japan
(Tel:0958-49-7649, Fax:0958-49-7650)
REFERENCE 2 (bases 1 to 3762)
AUTHORS Ohara,N.
JOURNAL Unpublished (1996)
REFERENCE 3 (sites)
AUTHORS De Wit,L., de la Cuvellerie,A., Ooms,J. and Content,J.
TITLE Nucleotide sequence of the 32 kDa-protein gene (antigen 85 A) of
Mycobacterium bovis BCG
JOURNAL Nucleic Acids Res. 18 (13), 3995 (1990)
MEDLINE 90326531
REFERENCE 4 (sites)
AUTHORS Ohara,N., Kitaura,H., Hotokezaka,H., Nishiyama,T., Wada,N.,
Matsumoto,S., Matsuo,T., Naito,M. and Yamada,T.
TITLE Characterization of the gene encoding the MPB51, one of the major
secreted protein antigens of Mycobacterium bovis BCG, and
identification of the secreted protein closely related to the
fibronectin binding 85 complex
JOURNAL Scand. J. Immunol. 41 (5), 433-442 (1995)
MEDLINE 95242057
COMMENT Sequence updated (24-Apr-1995) by:
Naoya Ohara.
FEATURES Location/Qualifiers
source 1..3762
/organism="Mycobacterium bovis"
/strain="BCG Tokyo"
RBS 535..541
CDS 547..1563
/citation=[2]
/citation=[3]
/codon_start=1
/product="32kDa protein (antigen 85 A)"
/db_xref="PID:g804883"
/transl_table=11
/translation="MQLVDRVRGAVTGMSRRLVVGAVGAALVSGLVGAVGGTATAGAF
SRPGLPVEYLQVPSPSMGRDIKVQFQSGGANSPALYLLDGLRAQDDFSGWDINTPAFE
WYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKWETFLTSELPGWLQANRHVKP
TGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAMGPTLIGLAMGDAGGYK
ASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWVYCGNGKPSDLGGNNLPAKFLEGFV
RTSNIKFQDAYNAGGGHNGVFDFPDSGTHSWEYWGAQLNAMKPDLQRALGATPNTGPA
PQGA"
sig_peptide 547..675
/codon_start=1
mat_peptide 676..1560
/codon_start=1
/product="32kDa protein (antigen 85 A)"
conflict replace(1612..1616,"cgta")
/citation=[2]
conflict replace(1697..1701,"ggtt")
/citation=[2]
conflict replace(1722..1731,"tacacgaacg")
/citation=[2]
RBS 1731..1736
conflict replace(1732..1736,"gaaggt")
/citation=[2]
sig_peptide 1743..1841
/codon_start=1
CDS 1743..2642
/citation=[3]
/codon_start=1
/product="MPB51"
/db_xref="PID:g804884"
/transl_table=11
/translation="MKGRSALLRALWIAALSFGLGGVAVAAEPTAKAAPYENLMVPSP
SMGRDIPVAFLAGGPHAVYLLDAFNAGPDVSNWVTAGNAMNTLAGKGISVVAPAGGAY
SMYTNWEQDGSKQWDTFLSAELPDWLAANRGLAPGGHAAVGAAQGGYGAMALAAFHPD
RFGFAGSMSGFLYPSNTTTNGAIAAGMQQFGGVDTNGMWGAPQLGRWKWHDPWVHASL
LAQNNTRVWVWSPTNPGASDPAAMIGQAAEAMGNSRMFYNQYRSVGGHNGHFDFPASG
DNGWGSWAPQLGAMSGDIVGAIR"
mat_peptide 1842..2639
/codon_start=1
/product="MPB51"
BASE COUNT 646 a 1207 c 1274 g 635 t
ORIGIN
1 aagtggtacc cgggtattcc cggctacatc gaccagcagt gggtcacgca agccgaagcg
61 gccctgcaat gccccgcgac tcgggcggtg cttaattctg tgcgcgcccc gatcaccctg
121 caccgattcc tctccaacgt gttgcattcc tacgagttca cccgttaccg catcgaccgc
181 gttccgcgct atgaattggt ccggtgtggg ctcgatgttc ccgacgggcc cggcccgcca
241 ccgcgggagt gaggggcaat gagcgcgggg gcaatactga cagcaagatc acaattgagc
301 cggcactagc ggtcgacaca tgcccagaca ctgcggaaat gccaccttca ggccgtcgcg
361 tcggtcccga attggccgtg aacgaccgcc ggataagggt ttcggcggtg cgcttgatgc
421 gggtggacgc ccgaagttgt ggttgactac acgagcactg ccgggcccag cgcctgcagt
481 ctgacctaat tcaggatgcg cccaaacatg catggatgcg ttgagatgag gatgagggaa
541 gcaagaatgc agcttgttga cagggttcgt ggcgccgtca cgggtatgtc gcgtcgactc
601 gtggtcgggg ccgtcggcgc ggccctagtg tcgggtctgg tcggcgccgt cggtggcacg
661 gcgaccgcgg gggcattttc ccggccgggc ttgccggtgg agtacctgca ggtgccgtcg
721 ccgtcgatgg gccgtgacat caaggtccaa ttccaaagtg gtggtgccaa ctcgcccgcc
781 ctgtacctgc tcgacggcct gcgcgcgcag gacgacttca gcggctggga catcaacacc
841 ccggcgttcg agtggtacga ccagtcgggc ctgtcggtgg tcatgccggt gggtggccag
901 tcaagcttct actccgactg gtaccagccc gcctgcggca aggccggttg ccagacttac
961 aagtgggaga ccttcctgac cagcgagctg ccggggtggc tgcaggccaa caggcacgtc
1021 aagcccaccg gaagcgccgt cgtcggtctt tcgatggctg cttcttcggc gctgacgctg
1081 gcgatctatc acccccagca gttcgtctac gcgggagcga tgtcgggcct gttggacccc
1141 tcccaggcga tgggtcccac cctgatcggc ctggcgatgg gtgacgctgg cggctacaag
1201 gcctccgaca tgtggggccc gaaggaggac ccggcgtggc agcgcaacga cccgctgttg
1261 aacgtcggga agctgatcgc caacaacacc cgcgtctggg tgtactgcgg caacggcaag
1321 ccgtcggatc tgggtggcaa caacctgccg gccaagttcc tcgagggctt cgtgcggacc
1381 agcaacatca agttccaaga cgcctacaac gccggtggcg gccacaacgg cgtgttcgac
1441 ttcccggaca gcggtacgca cagctgggag tactgggggg cgcagctcaa cgctatgaag
1501 cccgacctgc aacgggcact gggtgccacg cccaacaccg ggcccgcgcc ccagggcgcc
1561 tagctccgaa cagacacaac atctagcggc ggtgaccctt gtggtcgccg ccgctagatg
1621 tttcctaaat cccgtcccta gctcccgccg cgggccgtgt ggttagctac ctgacgggct
1681 aggggttggc cggggcggtc tgacgccggg tgcacacagc cttacacgac ggaggtggac
1741 acatgaaggg tcggtcggcg ctgctgcggg cgctctggat tgccgcactg tcattcgggt
1801 tgggcggtgt cgcggtagcc gcggaaccca ccgccaaggc cgccccatac gagaacctga
1861 tggtgccgtc gccctcgatg ggccgggaca tcccggtggc cttcctagcc ggtgggccgc
1921 acgcggtgta tctgctggac gccttcaacg ccggcccgga tgtcagtaac tgggtcaccg
1981 cgggtaacgc gatgaacacg ttggcgggca aggggatttc ggtggtggca ccggccggtg
2041 gtgcgtacag catgtacacc aactgggagc aggatggcag caagcagtgg gacaccttct
2101 tgtccgctga gctgcccgac tggctggccg ctaaccgggg cttggccccc ggtggccatg
2161 cggccgttgg cgccgctcag ggcggttacg gggcgatggc gctggcggcc ttccaccccg
2221 accgcttcgg cttcgctggc tcgatgtcgg gctttttgta cccgtcgaac accaccacca
2281 acggtgcgat cgcggcgggc atgcagcaat tcggcggtgt ggacaccaac ggaatgtggg
2341 gagcaccaca gctgggtcgg tggaagtggc acgacccgtg ggtgcatgcc agcctgctgg
2401 cgcaaaacaa cacccgggtg tgggtgtgga gcccgaccaa cccgggagcc agcgatcccg
2461 ccgccatgat cggccaagcc gccgaggcga tgggtaacag ccgcatgttc tacaaccagt
2521 atcgcagcgt cggcgggcac aacggacact tcgacttccc agccagcggt gacaacggct
2581 ggggctcgtg ggcgccccag ctgggcgcta tgtcgggcga tatcgtcggt gcgatccgct
2641 aagccaggtg ccgatgcgag ctgcctggcc gctatcggat gatccttgcg gcagacgtgc
2701 acgacatcgc gactcgggcg aagcgcgtag cgggccggta ccgtagaggg agtgcggcaa
2761 cgggcgggga tggccgcgct atcgcgcggc gcgggtgcac ccctttcgcg tgcgctaccg
2821 actctgttag caggagaaca tggccaagaa ctctcggcgt aagcgccacc gaatccttgc
2881 ctggatagcg gcgggcgcga tggcgtccgt cgtagcgctg gtcatcgtgg ccgtggtgat
2941 catgctgcgc ggcgccgaat cgccgcccag cgcggtgccg ccaggagttt taccaccggg
3001 gcccacccca gcacatccgc acaaaccccg accggccttc caggatgcgt cctgccccga
3061 cgtgcagatg atctcggttc ccggaacctg ggagtcatcg ccgcagcaga acccactgaa
3121 tccggtgcag tttccgaagg cgttgctgct caaggtaact gggccgatcg cccagcaatt
3181 cgcacccgcc cgggtgcaga cctacacagt tgcctacacg gcccaattcc acaatccgct
3241 gaccacggat aagtcacgat gtcgtacaac gacagccggg cagagggcac ccgcgcgatg
3301 gtggcagcga tgaccgatat gaacaacagg tgcccgttga ccagctatgt gctgatcggg
3361 ttttcgcagg gcgcggtgat cgcgggcgac gtggctagcg atatcggcaa cggccgggga
3421 cccgtggacg aggacctagt actaggcgtg acgctgatcg ccgacggtcg tcgtcagcag
3481 ggggtgggta atcaggtccc gcctagcccg cgcggcgaag gcgccgagat caccctgcac
3541 gaagtcccag tgttgtccgg gcttggtttg actatgaccg gcccgcggcc gggcggtttt
3601 ggtgcgctcg acggccggac caacgagatc tgcgcccagg gcgacctgat ctgtgcggcc
3661 ccggcacagg cgttcagtcc cgccaacttg ccgactacgc tgaacaccct ggccggcggc
3721 gccggccagc cggtccatgc tatgtatgcg acacccgaat tc
//